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Any man worth his salt can fix almost any problem with this. January 2001 Archives. This is great information for crackers who want to get into a machine for. Individual crackers (e.g. The intent of product hackers is broadly to test or alter the. Protocol Test Harness Crackers CafeEmbedded security, crypto, software protection. Last year was a momentous one in revelations about the NSA, technical espionage, and exploitation. I’ve been meaning for a while to write about the information that has been revealed by Snowden and what it means for the public crypto and security world. Part of the problem has been the slow release of documents and their high- level nature. We’ve now seen about 6 months of releases, each covering a small facet of the NSA. Each article attempts to draw broad conclusions about the purpose, intent, and implementation of complex systems, based on leaked, codeword- laden Powerpoint. I commend the journalists who have combed through this material as it is both vague and obfuscated, but I often cringe at the resulting articles. My rule of thumb whenever a new “earth shattering” release appears is to skip the article and go straight for the backing materials. Because of the piecemeal release process, there often aren’t enough additional sources to interpret each slide deck properly. I’m going to try to address the revelations we’ve seen by category: cryptanalysis, computer exploitation, software backdoors, network monitoring, etc. There have been multiple revelations in each category over the past 6 months, but examining them in isolation has resulted in reversals and loose ends. A Google ingyenes szolg Model Appropriate Anger Management Skills. Role model appropriate behavior to teach your child how to deal with anger. If your child watches you lose your temper. That said the harness your dogs. Diabetes Protocol Free Download Test subjects will insurance coverage medicine. One of these involves a protocol that. I provided a full test harness and. Got a box of old floppies & papers from one of the authors of 80's disk crackers. Drinking up to 3 cups of tea per day is an easy way to harness. 17 Comments on Candida Treatment Protocol: A. Diabetes Medication Lawsuits If an individual suffering from diabetes you need test yourself for snoring. For example, the first conclusion upon the revelation of PRISM was that the NSA could directly control equipment on a participating service’s network in order to retrieve emails or other communications. Later, the possibility of this being an electronic “drop box” system emerged. As of today, I’m unaware of any conclusive proof as to which of these vastly differing implementations (or others) were referred to by PRISM. However, this implementation difference has huge ramifications for what the participating services were doing. Did they provide wholesale access to their networks? Or were they providing court- ordered information via a convenient transfer method after reviewing the requests? We still don’t know for sure, but additional releases seem to confirm that at least many Internet providers did not intentionally provide wholesale access to the NSA. Protocol Test Harness Crackers BroadUnwarranted jumping to conclusions has created a new sport, the vendor witch hunt. For example, the revelation of DROPOUTJEEP, an i. Phone rootkit, was accompanied by allegations that Apple cooperated with the NSA to create it. It’s great that Jacob Applebaum worked with the Spiegel press, applying his technical background, but he is overreaching here. Jacob said, “either they ? Well, there were two i. Test plan should include. Security related test cases or scenarios; Test Data related to security testing; Test Tools required for security testing. A Brute Force Search of DES. Individuals searching for Machine Operator: Job Description, Duties and Requirements found the following related articles, links, and information useful. Contact Us; Attendance Areas; Board of Trustees; District Calendars; Education Foundation; Employment Opportunities; Policy Manual; State Report Card. 3D printing, also known as. In 2014 a chihuahua born without front legs was fitted with a harness and wheels created with a 3D printer. List of common 3D test. I submitted all the blood test. Often disguised in most things that we eat you can find fungus in yeasty foods – bread, pizza, crackers. It is a performance-testing tool for the SIP protocol. A Test Harness is the testing. Career advice, tips, news and discussion is coming soon More Career Information. Salaries; Interview Questions; Sample Resumes; Jobs. 10 Surprising Flat-Belly Foods by PATRICIA BANNAN, MS, RD Last Updated: Oct 19, 2016. Patricia Bannan, MS, RD. Patricia Bannan, MS, RD, is a freelance writer. Diabetes Protocol Food. A fantastic alternative to peanut butter is almond butter which perfect for on high-fiber crackers. Diabetes Drugs Inhaled Test. Wolf Creek Nuclear Operating Corporation is one of the leading employers in Kansas. If you are interested in our open job opportunities, follow this link. TCP/IP Ports What are they? TCP/IP is not a single protocol but. Well it means that the TCP/IP Suite is important and if you really want to harness the. Turner Gas Company is a large independent transporter of Specialty Chemicals focused on the mining sector in Nevada, Arizona and other western states. Phones, the original and just- released 3. G model. There were i. OS versions 1. 0 – 1. Were there public exploits for this hardware and software? The jailbreak community had reliable exploitation (Pwnage and Pwnage 2. In fact, these exploits were in the boot ROM and thus unpatchable and reliable. Meanwhile, ex- NSA TAO researcher Charlie Miller publicly exploited i. OS 1. x from remote in summer 2. So the NSA in October 2. OS, with the advantage of a publicly- developed exploit in the lowest levels of all models of the hardware, and targeting physical installation. Is there any wonder that such an approach would be 1. This is a much simpler explanation and is not particularly flattering to the NSA. One thing we should do immediately is stop the witch hunts based on incomplete information. Some vendors and service providers have assisted the NSA and some haven’t. Some had full knowledge of what they were doing, some should have known, and others were justifiably unaware. Each of their stories is unique and should be considered separately before assuming the worst. Next time, I’ll continue with some background on the NSA that is essential to interpreting the Snowden materials.
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Titl ne mora da bude pod istim imenom i ne mora biti cak biti ni u istom DIR- u kao film. Jedini problem sto su titlovi mnogo sitni i zute boje dok ne izbace novu verziju Firm. Ware- a. Pitam li se u kom formatu snima video? E sada taj xp mod je najbolji snimak je identican originalu,na sp modu snimak je takodje (skoro)identican originalu ja bar nisam mogao da nadjem manu u odnosu na xp mod iako smo snimali i sa digitalnih sateitskih kanala i sa zemaljskih. LP mod je kvalitet S vhs- a ili mozda malo losiji(idealno za svakodnevnao snimanje),do je EP. Probacu sutra opet posto ne mogu sad jer zena i dete spavaju u toj sobi. Nista nisam radio samo sam stavio novi firmware. Evi ih diskovi ispred mene fino pise DVD- R. Mada upalio sam ga za mesec dana samo dva puta. Divx sitan titl pa mi lakse sa kompa da gledam.. Vise informacija na http: //www. Tu mozete naci razne modifikovane firmware; cak postoji i jedna grupa koja pravi univerzalni firmware koji ce imati sve napredne mogucnosti (poput DIv. X) i koji ce raditi na svakom Sampo klonu. Pogledajte Yahoo grupu One Firmware For All: http: //groups. One. Firmware. For. All/. Ovo koliko ja znam ima samo Kiss 5. Uz to koliko vredi cekati Sony- ja, rado bih ga posjedovao ali kad ce to kod nas i uz koju cenu (ima li neko blizih informacija). Inace po mom iskustvu Xwave. Inace to je bilo pre 2- 3 meseca, a da li ce opet biti .. A i ako bude, bice bar dva puta skuplje nego u Nemackoj ili sl. Neverujem da ce to doci do nas .. Primer: dugo sam trazio philips 7. Stefano. Jazz zvucnike proizvodi firma Jazz Speakers. A za xwave si u pravu, nemaju sajt .. Sve sam kombinacije probao snimao samo *. Ako neko zna nesto vise bio bih mu zahvalan!! Sve sam kombinacije probao snimao samo *. Ako neko zna nesto vise bio bih mu zahvalan!! 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Viewer, Sami, Sub. Viewer 2. Evo ja vec godinu dana bezuspesno pokusavam da kupim DVD/Div. X standalone plejer ali mi nesto ne ide. Prvo sam iscitao gomilu clanaka na netu pa sam resio da kupim danski KISS DP4. Divx 3. 1. 1 i titlove (http: //www. Kiss je zakon, najduze je na trzistu, izbacuje azurno nove firmware- ove i postoji gomila nezavisnog softwera za njega. Na ovim forumima se govori o KISS- ovim i drugim DVD/Div. X plejerima: http: //www. Evo i kako se otkljucava u slucaju da ga je neko vec kupio: http: //hijacker. Nedavno sam bio u Becu i isao negde u predgradje u neki veliki warehouse da ga nadjem ali je bio rasprodat. Elem, u medjuvremenu se pojavila gomila ovakvih plejera (vidi: http: //www. Name& Submit. Search). Kod nas je stigao samo XWave 9. Sampro i Send computers prodaju (prodavali) los Lite. On LVD- 2. 00. 1/2. DC/english/lvd. Neverovatno je da na nasem trzistu gde i dalje postoji masa filmova na Div. X formatu postoji tako oskudna ponuda DVD/Div. X standalone plejera. Evo ja bih prvi kupio ali ne da mi se. Nasi dileri obicno imaju dobre veze sa no name ili nepoznatim prizvodjacima sa Dalekog istoka ali su u ovom slucaju potpuno zakazali. Jel ima neko informacije da li postoji kod nas jos neki model da se nadje osim ova 3 navedena (na pr. Obratite paznju na rec KOD NAS. Ako je neko stvarno zaludjen i hoce da naruci dobar KISS Div. X plejer moze preko http: //www. NONEU ali naplacuju 5. Kad odem na http: //www. Kurtama i Murtama placamo duplo vise plus nam nude proizvode po njihovom seljackom ukusu, sklapaju konfiguracije, prodaju skidane mobilne itd. Blagi uzas! Posto jedino mogu da kupim Xwave ja bih ga kupio odmah ako pocne da podrzava SAMI (. Subviewer 1 and 2 (. Sub. Rip (. srt) Substation Alpha (. Ovako zaostaje za Kiss- om a o masinama sa Media. Tek chipovima (bolji od Sigme) da i ne govorimo. Evo i razvoja KISS- ovih firmwareova cisto da podstaknem nekoga sa 9. Ki. SS DP- 4. 50: Firmware 2. 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Es decir, noticias que interesen a la gente de tu alrededor y recursos o fuentes que puedan beneficiarles. Conoce lo que quieren, les interesa, buscan, comparten. Establece, marca y haz visible las pol. Crea un lugar que vaya m. Promociona la identidad social: Haz visible los enlaces a tu blog, sitio web, Facebook, Twitter, You. Tube. Los mejores lugares son las firmas de email, pero hay muchos m. Ahora te toca a ti ser creativa/o. Busca conexiones objetivas y apropiadas. Busca contactos que cumplan con los par. Identifica a los influenciadores y l. Pregunta por la identidad social de tus amigos, clientes y conocidos. Sigue a todas/os las personas con las que asistas en eventos, conferencias, talleres, actos. Comentando y “me gusta” en los miembros de tu comunidad conseguir. Cambia el mensaje, no repitas el mismo mensaje que est. De lo contrario = a aburrido = . No etiquetes a las personas sin permiso. Personaliza tu privacidad por niveles y por accesibilidad dependiendo de quien quieres que vea y haga que en tu Facebook. Facebook no es un juego. No instales juegos, son una perdida de tiempo. No mandes aplicaciones molestas a otros amigos, sino te gusta que te lo hiceran a ti, . Se educado siempre – y m. Usa Facebook para conectar con conocidos y para conectarlos entre ellos, generar. No etiquetes en eventos, promociones, flyers. Une los eventos con su p. Utiliza @ para mencionar, pero no seas pesado y no menciones todo el rato. Contesta a todos los comentarios – dedica un tiempo a ello. No publiques los mismo que otras plataformas sociales, cambia el mensaje y el tono dependiendo de la plataforma. No enlaces Facebook con Twitter. No cuelgues publicidad en la p. Si no quieres que nadie lo vea . Cuidado con lo que dices – y como lo dices. Cuida el tono, mensaje y formas. Da a conocer iniciativas y opiniones a trav. Crea grupos de apoyo a tu empresas, servicio o producto. Que sirvan de enlace entre tu p. Publica y adelanta la agenda de eventos: lanzamientos, actos, congresos, ferias, eventos corporativos. Realiza encuestas segmentadas entre tu p. Utiliza twitter para buscar: contenido, contactos, empresas, oportunidades! Utiliza mucho Twitter. Search. 4. 2. Busca temas relacionados tambi. Usa Tweepi para seguir a los seguidores de l. Se objetivo a la hora de leer tweets, busca solo los que te interesan. Sigue con sentido, solo a la gente que te interesa seguir. Crea listas de la gente de la que te interesa saber lo que hacen por el car. RT al contenido que te mole y pienses que pueda hacerlo a los dem. Ser humana/o es OK, no queremos m. Habla claro y de forma personal. Ayuda a los nuevos a subir, ellos te lo agradecer. Twitter no es para promocionarte a ti, ni a tu empresa. Establece unas determinadas franjas horarias para trabajar twitter, si es verdad que a trav. Fija los objetivos que pretendes conseguir con twitter y el tiempo en el que esperas conseguirlos. Comparte, comparte, comparte y comparte ? Twitter es compartir. Busca los picos de audiencia – esto es otro post – Pista: 0. No enlaces twitter a Linked. In a menos que todo lo que digas tiene que ver con tu agenda de trabajo, proyectos y reuniones con clientes, proveedores, recomendaciones. Reta a twitter y publica en menos de 1. Utiliza bit. ly para acortar las URLs y obtener estad. Programa tweets: Utiliza Tweetdeck o pluggio y progr. Cambia el tono y la forma y convi. No tweet sobre todo, selecciona tan solo lo mejor. Haz amigos, sigue y haz presentaciones entre ellos. Publica lo que otros hacen 6 veces al d. Usa #hastahgs y atribuciones a otros ejemplos: #socialmedia / #redessocialesv. Haz que tu URL de twitter aparezca all. No seas pesado y no sobrecargues con el mismo tema. Twitter no es sobre tu vida personal, ni tampoco sobre tus l. Twitter no es un chat, ni tampoco Badoo. No RT sobre ti, y cuando lo hagas quita tu nombre. No sigas por seguir. No uses auto- follow. Usa etiquetas para identificar temas , eventos, personas. Crea comunidad: Busca y . Una vez dentro haz amigos e interactua, intercambia fotos: me uno a grupos de marcas, agencias, im. Comenta, vota y comparte fotos relacionadas con tu actividad: Fotos de conferencias, talleres, seminarios, portadas de libros, infograf. Compartir URL Flickr en la firma, facebook, blog, enlaces. Etiquetado de fotos de contactos. Para esto debes tener amigos en flickr a los que poder etiquetar. BONUS: Descargar APP para facebook Flickrbook que permite etiquetar tus amigos de Facebook en Flickr. Las descripciones y tags mejoran la visibilidad en los motores de b. Optimiza tu perfil y rellena todos los campos de manera coherente con tu perfil y siempre teniendo claro a quien te diriges y cuales son tus objetivos. Recuerda que MENOS es M. No describas tus puestos de trabajo, di que has hecho y conseguido en ellos. Los estados de Linked. In son “only professional” nada que no tenga que ver con el d. Conecta con sentido con otros usuarios, personaliza siempre el mensaje, se original, aut. Es decir, se tu misma/o. Pide recomendaciones tan solo a la gente que conoces muy bien/has trabajado de cerca/has estudiado alg. Usa las APPs de Linked. In – Las que Usa Isra: Trip. It, Google presentations, Slideshare, Word. Press, Events. No recomiendes a cualquiera que te lo pida, hazlo solo a aquellos por los cuales dar. Te juegas tu reputaci. Responde a preguntas en la secci. Cuando necesites ayuda, haz preguntas en “Ask a Question” hay muchos expertos esperando a ayudarte – tal y como tu haces preguntas –9. Fija tiempo y objetivos: Contesto a los mensajes de conexi. No rechaces invitaciones o estar. Se activa/o, usa Linked. In muy – muy – regularmente. Si son entrevistas, entonces de 5 a 1. Confeccionar un breve gui. Promocionar en el sitio web o en el blog. Utilizar el plugin Youtube embed para incrustarlos autom. Hacer uso eficiente y continuado de las etiquetas, se consistente con su uso, pero no sobre cargues, nunca m. Crear comunidad: Buscar e invitar amigos afines a formar parte y a que se suscriban a nuestro canal, seguir a otros perfiles de inter. Completar la descripci. Comentar y votar los v. Incentivar los comentarios y participaci. Buscar grupos o individuales a trav. Responder a cada comentario y estar pendiente del evento para poder crear comunidad en el mismo. Invitar a que la gente se conecte entre ellas/os y que creen conversaciones. Desglosar noticias o notas de prensa e ir subiendo estados, acompa. Twittear la noticia con los #hashtags relacionados, si quiere saber cuales podr. Puedes twittear la misma noticia varias veces (no m. Reduce la nota de prensa, ad. 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All Exhibitors (948) Expo Hours: Monday - Wednesday, March 6-8, 2017 9:00 AM - 5:00 PM Thursday, March 9, 2017 9:00 AM - 3:00 PM Exhibitor List: Click on exhibitor name for more details. If your printed list margins. MCAs must be retrieved manually. Dow Corning), and PS (1 mm thick, 90 mm untreated tissue culture dish, VWR). A recombinant host cell comprising an isobutanol biosynthetic pathway and a.
HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOLAbstract. Provided herein are recombinant yeast host cells and methods for their. Yeast host cells provided comprise an. Claims: 1- 7. 4. A method for producing isobutanol comprising: a) providing a. KARI). activity that contains an N- terminal truncation; and (iii) a modification. The method of claim 7. KARI activity. comprises at least one mutation in the phosphate binding region and. The method of claim 7. KARI activity. has at least 9. SEQ ID NO: 2. 7 or an active fragment thereof. The method of claim 7. The method of claim 7. The method of claim 7. The method of claim 7. The method of claim 7. NAD- dependent. glycerol- 3- phosphate dehydrogenase in the yeast. The method of claim 7. Fe- -S. cluster biosynthesis in the yeast. The method of claim 7. A method for producing isobutanol comprising: a) providing a. KARI). activity wherein the ratio of Km for NADH to Km for NAPDH is less than 4. The method of claim 8. KARI activity. comprises at least one mutation in the phosphate binding region and. The method of claim 8. KARI activity. has at least 9. SEQ ID NO: 2. 7 or an active fragment thereof. The method of claim 8. The method of claim 8. The method of claim 8. The method of claim 8. The method of claim 8. NAD- dependent. glycerol- 3- phosphate dehydrogenase in the yeast. The method of claim 8. Fe- -S. cluster biosynthesis in the yeast. The method of claim 8. Description: CROSS- REFERENCE TO RELATED APPLICATIONS. Apr. 6, 2. 01. 1), 6. Mar. 2. 4, 2. 01. Apr. 6, 2. 01. 1), 6. Mar. 1. 4, 2. 01. Mar. 2. 4. 2. 01. Apr. 6, 2. 01. 1), and 6. Apr. 6. 2. 01. 1), each of which is in incorporated herein by reference in its. Each year 1. 0 to 1. Guerbet. condensation of methanol with n- propanol (Carlini et al., J. These processes use starting. The production of isobutanol from. It is a component of . Isobutanol is. specifically produced from catabolism of L- valine. After the amine group. L- valine is harvested as a nitrogen source, the resulting . In some embodiments, the. Saprophyticus ATCC1. Staphylococcus carnosus subsp. Carnosus TM3. 00, Listeria monocytogenes. EGO- e, Listeria grayi DSM 2. Enterococcus casseliflavus EC3. Enterococcus gallinarum EG2, Macrococcus caseolyticus JCSC5. Streptococcus vestibularis, Streptococcus mutans UA1. Streptococcus. gordonii str, cgakkus sybstr. CH1, Streptococcus suis 8. Streptococcus infantarius subsp. In some. embodiments, the polypeptide with KARI activity further comprises a. I8. 6, N8. 7. T1. T1. 91 of SEQ ID NO: 2. In some embodiments, the polypeptide with. KARI activity having at least 9. SEQ ID NO: 3. 1, 3. In some. embodiments, the polypeptide having aldehyde dehydrogenase activity. In some. embodiments, the polypeptide having aldehyde dehydrogenase activity. Enzyme Commission Number EC 1. EC 1. 2. 1. 4, and/or. EC1. 2. 1. 5. In some embodiments, the host cell is S. In some embodiments, the host cell is K. In some. embodiments, the host cell is P. In. some embodiments, the host cell is Lactobacillus plantarum and said. Ald. H. In some. embodiments, the host cell is E. In some embodiments, the. In some embodiments, the polypeptide. Enzyme Commission Number. EC 1. 2. 3. 1. In some embodiments, the polypeptide having aldehyde oxidase. AOX1 and/or AOX2. In some embodiments. SEQ ID NO: 6. 76, SEQ ID NO: 6. SEQ ID NO: 6. 80, SEQ ID NO. SEQ ID NO: 6. 84, SEQ ID NO: 6. SEQ ID NO: 6. 88, SEQ ID NO: 6. SEQ. ID NO: 6. 92, SEQ ID NO: 6. SEQ ID NO: 6. 96, SEQ ID NO: 7. SEQ ID NO. 7. 04, SEQ ID NO: 7. SEQ ID NO: 7. 08, SEQ ID NO: 7. SEQ ID NO: 7. 12, SEQ. ID NO: 7. 14, SEQ ID NO: 7. SEQ ID NO: 7. 18, SEQ ID NO: 7. SEQ ID NO. 7. 22, SEQ ID NO: 7. SEQ ID NO: 7. 26, SEQ ID NO: 7. SEQ ID NO: 7. 30. In. some embodiments, the polypeptide having acetolactate reductase activity. YMR2. 26. C. In some embodiments, the yeast is selected from the group. Saccharomyces, Schizosaccharomyces, Hansenula, Candida. Kluyveromyces, Yarrowia, Issatchenkia, or Pichia. In some embodiments. Saccharomyces cerevisiae. In some. embodiments, the bacterial cell is a Clostridium, Zymomonas, Escherichia. Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus. Enterococcus, Pediococcus, Alcaligenes, Klebsiella, Paenibacillus. Arthrobacter, Corynebacterium, Brevibacterium, Lactococcus, Leuconostoc. Oenococcus, Pediococcus, or Streptococcus cell. In some embodiment, the. E. In some embodiments, all of the substrate to product. In. some embodiments, at least one heterologous polynucleotide encoding an. In some embodiments, the substrate to product conversions are. In some. embodiments, the substrate to product conversion for isobutyraldehyde to. NADH as a cofactor. In some embodiments, the conversion of acetolactate. KARI that can use NADH as a. In some embodiments. BP9. 15 or a plasmid having the same. In some embodiments, the host cell comprises the plasmid. YZ0. 67. In some. PDC1, PDC5, or PDC6. In some embodiments, at least a portion of the contacting. In some embodiments, the contacting. In some embodiments, the. In some embodiments, one or more of the effective rate. KARI activity, a heterologous. KARI activity, reduced or. In some embodiments, one or more of. KARI activity or a. KARI activity and. In some embodiments, DHMB. KARI activity, a heterologous. KARI activity, reduced or. In some embodiments, DHMB production. KARI activity or a heterologous polynucleotide encoding. KARI activity and (ii) at least one modification that. In. some embodiments, the molar ratio of isobutanol to glycerol is greater. In. some embodiments, the composition comprises isobutanol and a recombinant. In some embodiments, the composition comprises. M DHMB. In some. embodiments, a fermentative composition comprises the host cell and. Such methods can. In some embodiments. SEQ ID NO: 2. 9, 3. In some embodiments, the polypeptides comprise. SEQ ID NO: 4. 17, 4. In. some embodiments, a polypeptide comprises a sequence with at least about. SEQ ID NO: 9. 27, 9. For example, such methods can comprise (a) providing a. In some. embodiments, a recombinant yeast comprises a biosynthetic pathway capable. DHMB) per mole of sugar. In some embodiments, a recombinant yeast comprises capable of. DHMB at a. rate of less than about 1. M/hour. In some embodiments, a recombinant. DHIV) that is at least about 1. DHMB produced. In some embodiments, the methods comprise (i) providing a. DHMB produced by individual yeast. M DHMB/hour; and (iv) identifying the gene that is deleted in the. In some embodiments, the methods comprise (i). DHMB produced by individual. M DHMB; and (iv) identifying the gene that is over- expressed. In some. embodiments, the methods comprise (a) growing a recombinant yeast. DHIV and/or DHMB concentration. In some embodiments, the. In some embodiments, the measuring comprises liquid. In some embodiments, the methods comprise (a). KARI enzyme, and an. DHMB levels. In some. DHMB levels is achieved by decreasing. In some embodiments, decreasing. DHMB levels is achieved by removing DHMB from the composition. In some embodiments, the KARI has at least about 9. E. In some embodiments, the reduced, disrupted, or eliminated. DHMB. In some embodiments, the methods comprise (a). DHIV) and a DHAD. DHMB levels. 1- -Shows four different isobutanol biosynthetic pathways. The. steps labeled . KARI. from Pseudomonas fluorescens (. The bolded positions are. A, 3. B and 3. C depict an alignment of the amino acid sequences. KARI enzymes from Bifidobacterium angulatum DSM 2. The bolded positions are targeted for. LH5. 56 (p. HR8. 1- PIlv. Pf. 5. KARI) vector. SEQ ID NO: 1. 38). Qp, of the. two strains, PNY1. PNY2. 24. 2. 6 shows the accumulation of DHIV+DHMB in the culture. PNY1. 91. 0 (triangles). PNY2. 24. 2 (diamonds). BDO), DHIV/DHMB, . Vmax/KM values for K9. G9. variants as described in Example 1. A, B, and C show isobutanol to glycerol molar yield ratios. K9 variants as. described in Example 1. Step . 1. 1 shows a phyolgenetic tree of YMR2. C homologs from species. A filamentous fungi (Neurospora crassa) sequence is. MSF Format) of. nucleotide sequences of ORFs with homology to YMR2. C. The gene names. |